cell culture u937 cell lines  (Pasteur Institute)

Journal: Leukemia research

Article Title: Differentiation therapy in poor risk myeloid malignancies: Results of companion phase II studies

doi: 10.1016/j.leukres.2016.09.003

Figure Lengend Snippet: A Relative expression of CD11b and CD14 of U937 cells detected by flow cytometry following treatment with entinostat (ENT) with or without GM-CSF. B. Relative expression of CD11b by U937 or HL60 cells following treatment with bexarotene (BEX) with or without GM-CSF. C. Relative clonogenic growth of U937 or HL60 cells following treatment with ENT or BEX with or without GM-CSF. Values represent mean ± SEM of 3 experiments. *P < 0.05.

Article Snippet: Cell culture Human cell lines U937 and HL-60 were obtained from American Type Culture Collection (Manassas, VA) and maintained in RPMI 1640 (Life Technologies, Inc., Rockville, MD) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Life Technologies, Inc.), 50 units/ml penicillin, 50 μg/ml streptomycin, and 2 mM L -glutamine in a humidified atmosphere at 37 ◦C and 5.0% CO 2 .

Techniques: Expressing, Flow Cytometry

Journal: The Journal of Biological Chemistry

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses

doi: 10.1074/jbc.RA118.005676

Figure Lengend Snippet: Production of soluble Siglec-14 is not proportional to the expression level of membrane-bound Siglec-14. A, ELISA of soluble Siglec-14 produced by FLAG-Siglec-14/THP-1 and U937 cell lines. Siglec-14–specific mouse mAb (clone 40-1) was used for detection. B, expression levels of membrane-bound Siglec-14 on FLAG-Siglec-14/THP-1 and U937 cell lines. Solid line, cells stained with anti-Siglec-14 antibody (clone 40-1); gray-shaded area, cells stained with control mouse antibody.

Article Snippet: Cell lines and culture U937 human histiocytic lymphoma cell line (CRL-1593.2; American Type Culture Collection), the THP-1 acute monocytic leukemia cell line (obtained from Human Science Research Resources Bank, Osaka, Japan; now part of the JCRB Cell Bank at the National Institutes of Biomedical Innovation, Health and Nutrition, Japan), and their derivatives were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (pen/strep).

Techniques: Expressing, Membrane, Enzyme-linked Immunosorbent Assay, Produced, Staining, Control

Journal: The Journal of Biological Chemistry

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses

doi: 10.1074/jbc.RA118.005676

Figure Lengend Snippet: Human bone marrow cells produce alternatively spliced mRNA encoding soluble Siglec-14. Human bone marrow first-strand cDNA library (A–C) and first-strand cDNA prepared from U937 cells (C) were used as templates. A, agarose gel electrophoresis of 3′RACE PCR products obtained using a gene-specific primer (annealing to exon 5) and a universal primer. Products of nested PCR (second round) were separated by agarose gel electrophoresis. B, agarose gel electrophoresis of RT-PCR products with the primer pair flanking intron 5. C, relative abundances of membrane-bound (solid bars) and soluble (open bars) SIGLEC14 mRNA isoforms, normalized against ACTB mRNA.

Article Snippet: Cell lines and culture U937 human histiocytic lymphoma cell line (CRL-1593.2; American Type Culture Collection), the THP-1 acute monocytic leukemia cell line (obtained from Human Science Research Resources Bank, Osaka, Japan; now part of the JCRB Cell Bank at the National Institutes of Biomedical Innovation, Health and Nutrition, Japan), and their derivatives were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (pen/strep).

Techniques: cDNA Library Assay, Agarose Gel Electrophoresis, Nested PCR, Reverse Transcription Polymerase Chain Reaction, Membrane